Application of flow cytometry to industrial microbial bioprocesses

作者：Diaz Mario^*; Herrero Monica; Garcia Luis A; Quiros Covadonga

来源：Biochemical Engineering Journal, 2010, 48(3): 385-407.

DOI：10.1016/j.bej.2009.07.013

• Flow cytometry
• Instrumentation
• Cell parameters
• Bioprocesses control
• Optimization
• Kinetic modelling

Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture, by means of light-scattering and fluorescence measurements. In biotechnology, the power of this method lies both in the possibility of determining a wide range of cell parameters at single cell level, and in the ability to obtain information about their distribution within cell populations, providing valuable information for bioprocess design and control. The first part of this review will be devoted to describe the basic aspects of multicolour FC, the different strategies used for cell detection, and the cellular target sites and fluorescent probes which are currently used in cell assays. Next, a deep revision concerning the most recent FC applications related to cell analysis and quantification (especially both bacteria and yeast) in food, drink and pharmaceutical industries and in natural environment and water systems is presented. This review seeks to highlight the advantages of this technique in microbial fermentations monitoring and control, as well as in the development of more accurate kinetic models directed to bioprocesses optimization. Finally, an overview of some commercial FC devices is presented along with a discussion of the progress and advantages of the method for bioprocesses on-line monitoring.

• 出版日期2010-2-15