A Method to Evaluate Isotopic and Energy Turnover Rates in Larval Culex quinquefasciatus (Diptera: Culicidae) Using Stable Isotope Labeled Compounds

作者：Healy Kristen^*

来源：Journal of Medical Entomology, 2018, 55(4): 817-824.

DOI：10.1093/jme/tjy025

• N-15-labeled potassium nitrate
• C-13-labeled glucose
• C-13-labeled leucine
• larval density
• temperature

The goal of this study was to evaluate the use of stable isotope labeled compounds to better understand factors influencing energy turnover in larval Culex quinquefasciatus (Say; Diptera: Culicidae). Three isotope labeled compounds were evaluated in this study, including N-15-labeled potassium nitrate, C-13-labeled glucose, and C-13-labeled leucine. Conditions were first optimized in the laboratory to determine the most appropriate concentration of isotope, as well as the half-life of enrichment. Once optimum conditions were established we used standard equations to predict and determine temperature and density-dependent energy turnover rates. Our results showed that higher concentrations of isotope had an impact on mosquito survivability, overall enrichment, and adult wing length. We predicted the half-life of to be around 0.614 to 0.971 d, and our observed half-lives were determined to be 0.72 to 1.44 d depending on temperature, larval density, and isotope compound. Both density and temperature had a strong influence on isotopic turnover rates in all isotopes evaluated. Our results suggest that stable isotopes can provide a useful tool in understanding how different stress factors influence energy turnover in larval Cx. quinquefasciatus. These data can also help lay a foundation on ways to improve larvicide efficacy under different biotic and abiotic conditions.

• 出版日期2018-7