Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach

作者：Chen Han Min; Lin Ching Yu; Wang Vinchi^*

来源：Clinical Biochemistry, 2011, 44(5-6): 377-385.

DOI：10.1016/j.clinbiochem.2011.01.002

• Proteomics
• Parkinson's disease (PD)
• Two-dimensional electrophoresis (2-DE)
• Mass spectrometry (MS)
• Immunoglobulin G kappa light chain (IgG kappa L)
• Serum amyloid P component (SAP)

Objectives: Parkinson's disease (PD) ranks the second among the neurodegenerative disorders. Proteins involved in Parkinson's disease (PD) have been investigated but none as the diagnostic markers in blood.
Design and methods: In this study, we applied a proteomic strategy, by utilizing two-dimensional electrophoresis and mass spectrometry, to analyze two sample pools of plasma from the healthy individuals and PD subjects.
Results: IgG kappa L and human serum amyloid P component (SAP) were found differentially expressed between these pools. SAP level increased by approximately 5-fold in PD samples, and the ELISA procedure revealed a significant (P < 0.001) increase in SAP concentration (65.9 +/- 18.7 mu g/mL) in the plasma of PD subjects (healthy individuals, 35.0 +/- 12.5 mu g/mL), with sensitivity of 94.1% and specificity of 87.5%.
Conclusion: Our results indicated a potential feasibility of plasma SAP as a marker to approach PD.

• 出版日期2011-4