A novel long noncoding RNA OECC promotes colorectal cancer development and is negatively regulated by miR-143-3p

作者:Huang, Fengting; Wen, Chuangyu; Zhuansun, Yongxun; Huang, Lanlan; Chen, Wenying; Yang, Xiangling; Liu, Huanliang*
来源:Biochemical and Biophysical Research Communications, 2018, 503(4): 2949-2955.
DOI:10.1016/j.bbrc.2018.08.075

摘要

Emerging evidence indicates that aberrant long non-coding RNA (lncRNA) expression contributes to CRC pathogenesis. To explore the biological functions of IncRNAs in CRC and to identify the underlying mechanisms, we first conducted a IncRNA microarray assay to investigate lncRNA expression patterns in CRC. We identified a novel lncRNA OECC, originating from chromosome 8q24 that is highly expressed in CRC tissues and cell lines and has a positive correlation with liver metastasis. Attenuation of IncRNA OECC expression prohibited CRC cell proliferation, induced apoptosis, and inhibited migration. Furthermore, an inverse correlation between lncRNA OECC and miR-143-3p was observed. Bioinformatic analyses predicted, and a luciferase reporter assay demonstrated, that IncRNA OECC is a direct target of miR-143-3p, leading to down-regulation in the expression of its target genes, the NF-kappa B and p38 MAPK pathways. Taken together, our results suggest that IncRNA OECC is overexpressed in CRC and may play an oncogenic role through NF-kappa B and p38 MAPK pathway activation via miR-143-3p.