A sensitive immunosorbent bio-barcode assay combining PCR with icELISA for detection of gonyautoxin 2/3

作者：Tang, Yong; Wang, Hong; Xiang, Junjian^*; Chen, Yaoqiang; He, Wenfei; Deng, Ning; Yang, Hongyu

来源：Analytica Chimica Acta, 2010, 657(2): 210-214.

DOI：10.1016/j.aca.2009.10.045

• Gonyautoxin 2/3
• Anti-GTX 2/3 monoclonal antibodies
• Gold nanoparticles
• Bio-barcode DNA

In the current study, we developed a nanosphere bio-barcode technology to detect trace gonyautoxin 2/3 (GTX 2/3). GTX 2/3-glucose oxidase (GOX) conjugates were first prepared as the coating antigen in a periodate reaction. Subsequently, gold nanoparticles (NP) dual-labeled with anti-GTX 2/3 monoclonal antibodies (Mab) and DNA oligonucleotides were synthesized via a one-step preparation method. Combining PCR with indirect competitive ELISA (icELISA), a novel immunosorbent bio-barcode assay was established utilizing the Mab-NP-dsDNA complex to convert enzymatic signals to DNA signals. Importantly, the limit of detection of the method was lower than 0.74 mu g mL(-1). Thus, the immunosorbent bio-barcode assay is a rapid and high-throughput screening tool to detect GTX 2/3 in aquatic products.

• 出版日期2010-1-11
• 单位暨南大学