A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

作者：Mehta Radhika; Qureshi Munaum H; Purchal Meredith K; Greer Sylvester M; Gong Shanzhong; Ngo Chinh; Que Emily L

来源：Chemical communications, 2018, 54(43): 5442-5445.

DOI：10.1039/c8cc02034e

We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo-CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms.

• 出版日期2018-5-28