Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

作者：Tran Vy; Poole Daniel S; Jeffery Justin J; Sheahan Timothy P; Creech Donald; Yevtodiyenko Aleksey; Peat Andrew J; Francis Kevin P; You Shihyun; Mehle Andrew^*

来源：Viruses-Basel, 2015, 7(10): 5319-5327.

DOI：10.3390/v7102873

• influenza virus
• reporter virus
• multi-modal imaging
• multiplicity reactivation

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.

• 出版日期2015-10