Downregulation of active IKK beta by Ro52-mediated autophagy

作者：Niida Motoko; Tanaka Makoto; Kamitani Tetsu^*

来源：Molecular Immunology, 2010, 47(14): 2378-2387.

DOI：10.1016/j.molimm.2010.05.004

• Autophagy
• Trafficking
• Monoubiquitination
• Protein degradation
• Inflammation

Upon activation NF-kappa B translocates into the nucleus and inmates many biological events This NF-kappa B signaling is mainly induced by the protein kinase IKK beta Early in this signaling pathway IKK beta is phosphorylated for activation by several factors such as pro-inflammatory cytokines and the Tax oncoprotein of human T-cell leukemia virus type 1 (HTLV-1) In cells expressing Tax protein IKK beta is persistently phosphorylated which chronically activates NF-kappa B signaling But the active IKK beta is conjugated with a monoubiquitin by the E3 ubiquitin ligase Ro52 and the IKK beta-induced NF-kappa B signaling is downregulated However the mechanism of the downregulation has been unknown Here we show that Ro52-mediated monoubiquitination is involved in the subcellular translocation of active IKK beta to autophagosomes Furthermore using reporter assays we show that Ro52 suppresses IKK beta-induced NF-kappa B signaling and that this suppression is blocked by an au

• 出版日期2010-8