Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK

作者：Back Hyun moon; Song Byungjeong; Chae Jung woo; Yun Hwi yeol; Ma Jin Yeul^*; Kwon Kwang il

来源：Journal of Analytical Methods in Chemistry, 2015, 2015: 431632.

DOI：10.1155/2015/431632

KIOM-MA128 is a novel Korean herbal medicine with antiatopic, anti-inflammatory, and antiasthmatic effects. Matrine is thought to be a potential chemical marker of KIOM-MA128, but pharmacokinetic studies on KIOM-MA128 had not been performed. This study describes a simple and rapid method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to determine the concentration of matrine in rats plasma after administration of KIOM-MA128. The isocratic mobile phase consisted of methanol and distilled water, and the flow rate was 0.15 mL/min. The accuracy and precision of the assay, as well as stability tests, were performed in accordance with FDA regulations for the validation of bioanalytical methods. The half-life and T-max of matrine after administration of KIOM-MA128 were 4.29 +/- 2.20 h and 1.8 +/- 1.23 h, respectively. C-max and AUC(inf) of matrine after administration of KIOM-MA128 at 4 g/kg and 8 g/kg were 595.10 +/- 182.91 ng/mL, 5336.77 +/- 1503.84 ng/mL.h and 850.46 +/- 120 ng/mL, 9583.10 +/- 888.92 ng/mL.h, respectively. The validated method was successfully applied to a pharmacokinetic study in rats after oral administration of KIOM-MA128.

• 出版日期2015