Wood characteristics and enzymatic saccharification efficiency of field-grown transgenic black cottonwood with altered lignin content and structure

作者：Xiang Zhouyang; Sen Suman Kumar; Roy Aparna; Min Douyong; Savithri Dh****ekshmi; Jameel Hasan^*; Chiang Vincent; Chang Hou min

来源：Cellulose, 2015, 22(1): 683-693.

DOI：10.1007/s10570-014-0541-7

• Black cotton wood
• Enzymatic saccharification
• Field-grown
• Lignin
• Pretreatment
• S/V ratio
• Sugar yield
• Transgenic

The impact of field-grown transgenic trees on wood characteristics and enzymatic saccharification was evaluated. Genes 4CL and CAld5H were down regulated or overexpressed to modify the lignin content or structure (S/V ratio) on black cottonwood (Populus trichocarpa Nisqually-1). These transgenic trees were grown in the real environment and evaluated after 2 and 3 years. The results exhibited that the lignin content for most of the transgenic black cottonwoods increased compared to their initial lignin content when grown inside the greenhouse. The lignin contents approached those of the wild types. Acid and green-liquor pretreatments were applied to the transgenic wood saw dust samples. Cellulase mediated saccharifications were then conducted for the pretreated and untreated samples. Sugar yield and carbohydrate saccharification efficiency of the enzymatic saccharification for most of the transgenic trees were observed to be higher than those of the wild types even though their lignin contents became similar.

• 出版日期2015-2