科研之友
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摘要
In this study, a colorimetric biosensor was established for the detection of protein tyrosine phosphatase 1B (PTP1B) and its inhibitor based on the guanidinium recognition assisted coordination of arginineglycine-cysteine (RGC) modified gold nanoparticles (RGC/AuNPs) with 4-aminophenyl phosphate-functionalized Fe3O4 magnetic nanoparticles (MNPs/APP). PTP1B can catalyze the cleavage of the phosphate ester bond and the departure of the phosphate acid group from the surface of MNPs/APP, resulting in the loss of the corresponding coordination reactivity. Upon the addition of a solution of RGC/AuNPs, RGC/AuNPs remained in the supernatant solution after magnetic separation and a high absorbance value was observed. Thus, a simple colorimetric biosensor for PTP1B assay was developed. Under the optimized experimental conditions, PTP1B was detected within a linear range of 0.002 U mL(-1) to 0.08 U mL(-1) with the lowest detection limit of 0.0013 U mL(-1). Moreover, using this proposed biosensor, the inhibition effect of betulinic acid and 6-chloro-3-formyl-7-methylchromone on PTP1B is determined with IC50 values of 9 mu M and 15 mu M, respectively. Therefore, this new biosensor not only has great potential for PTP1B analysis but also for the detection of its inhibitors.
- 出版日期2017-12-7
- 单位上海大学
