Development of species-specific primers for identifying Auricularia auricula-judae using intergenic spacer 1 (IGS1) sequences

作者：Li, Li; Liu, Wei; Bian, Yin-Bing; Xiao, Yang^*

来源：African Journal of Biotechnology, 2011, 10(69): 15494-15500.

DOI：10.5897/AJB11.2433

• Auricularia
• Auricularia auricula-judae
• intergenic spacer
• species-specific primer

Auricularia auricula-judae, an important edible and medicinal mushroom was first cultivated in China more than one thousand years ago. In this study, we developed a simple and rapid polymerase chain reaction (PCR)-based diagnostic tool for identifying A. auricula-judae from other morphologically similar Auricularia species. Based on the sequence analysis of intergenic spacer 1 (IGS1) in genus Auricularia, two species-specific primer combinations were designed from IGS1 sequence of A. auricula-judae, both of them can generate DNA fragments of different size unique to A. auricula-judae. The validity of this two primer combinations were confirmed by correctly amplifying species-specific fragments from genomic DNA of another 32 A. auricula-judae strains. To our knowledge, this was the first study on development of species-specific primers via IGS1 sequence in jelly fungi and also the first application of IGS1 sequence in genus Auricularia.

• 出版日期2011-11-7
• 单位华中农业大学