Degradation of phytosterols in tobacco waste extract by a novel Paenibacillus sp.

作者：Ye, Jianbin; Zhang, Zhan; Yan, Ji; Hao, Hui; Liu, Xiangzhen; Yang, Zongcan; Ma, Ke; Yang, Xuepeng; Mao, Duobin; Zhou, Hao^*

来源：Biotechnology and Applied Biochemistry, 2017, 64(6): 843-850.

DOI：10.1002/bab.1547

• Paenibacillus sp
• phytosterol degradation
• tobacco waste extract
• HPLC-MS/MS

Phytosterols have been demonstrated to be precursors of polycyclic aromatic hydrocarbons (PAHs) formed during biomass pyrolysis. Here, a novel Paenibacillus sp. was evaluated for its ability to degrade phytosterols in tobacco waste extract (TWE). The optimal conditions for cell growth and stigmasterol (a representative of phytosterols) degradation were 37 degrees C, pH 7.0, 1.0g/L yeast extract, and 6.0g/L glucose. Paenibacillus sp. could degrade stigmasterol under high concentrations of glucose (up to 130g/L) and tolerate wide pH (5.0-9.0) and temperature (25-42 degrees C) ranges. The new strain could degrade stigmasterol completely into CO2 and H2O, and no intermediate steroids were detected during the degradation process. Phytosterol degradation in TWE was demonstrated by high-performance liquid chromatography-tandem mass spectrometry. Under optimal conditions (37 degrees C, pH 7.0, with the exponential-phase cells), the total degradation ratio of phytosterols reached 38.5% in TWE, including 45.2% of stigmasterol, 37.4% of -sitosterol, 27.3% of campesterol, and 28.7% of cholesterol. These results showed that Paenibacillus sp. is a candidate for phytosterol degradation in TWE and other biomass and is potentially useful in reducing the PAHs generated from biomass pyrolysis.

• 出版日期2017-12
• 单位郑州轻工业大学