Protective effects of alpha-pinene in mice with cerulein-induced acute pancreatitis

作者：Bae Gi Sang; Park Kyoung Chel; Choi Sun Bok; Jo Il Joo; Choi Mee Ok; Hong Seung Heon; Song Kyung; Song Ho Joon; Park Sung Joo^*

来源：Life Sciences, 2012, 91(17-18): 866-871.

DOI：10.1016/j.lfs.2012.08.035

• Alpha-pinene
• Acute pancreatitis
• Cerulein
• Cytokines

Aims: Acute pancreatitis (AP) is a complicated inflammatory disease that has an unknown underlying pathogenesis. Because alpha-pinene can modulate inflammation, we examined whether alpha-pinene plays a role in AP.
Main methods: Alpha-pinene was administered intraperitoneally 1 h prior to the first injection of cerulein. Once AP developed, cerulein, a stable cholecystokinin analog, was injected hourly over a 6-h period. Blood samples were taken 6 h later to determine serum amylase and lipase levels. The pancreas and lungs were rapidly removed for morphological examination, myeloperoxidase assay, and real-time reverse transcription polymerase chain reaction. We also isolated the pancreatic acinar cells using a collagenase solution. Cell viability, and cytokine productions were measured in pancreatic acini.
Key findings: Intraperitoneal administration of alpha-pinene reduced the pancreatic weight (PW) to body weight (BW) ratio and the serum levels of amylase and lipase. Alpha-pinene treatment also reduced histological damage and myeloperoxidase activity in the pancreas and lungs. Furthermore, alpha-pinene pretreatment reduced the production of pancreatic tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 during cerulein-induced AP. In vitro, alpha-pinene inhibited cerulein-induced cell death and cytokine production in isolated cerulein-treated pancreatic acinar cells.
Significance: These findings suggest that alpha-pinene has an anti-inflammatory effect during cerulein-induced AP.

• 出版日期2012-10-29