Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor

作者：Tachi Tomoya; Kaji Noritada; Tokeshi Manabu^*; Baba Yoshinobu

来源：Analytical Sciences, 2009, 25(2): 149-151.

DOI：10.2116/analsci.25.149

We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 mu g mL(-1). Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.

• 出版日期2009-2