Novel method for efficient production of multipotential hematopoietic progenitors from human embryonic stem cells

作者：Ma Feng; Wang Dan; Hanada Sachiyo; Ebihara Yasuhiro; Kawasaki Hirohide; Zaike Yuji; Heike Toshio; Nakahata Tatsutoshi; Tsuji Kohichiro^*

来源：International Journal of Hematology, 2007, 85(5): 371-379.

DOI：10.1532/IJH97.06203

• hematopoietic progenitors
• human embryonic stem cells
• fetal liver
• stromal cells

We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitors in coculture, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial cell potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type beta-globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. These progenitors showed neither the immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. ne coculture system developed for hESC can provide a novel source of hematopoietic and blood cells for applications in cellular therapy and drug screening.

• 出版日期2007-6
• 单位河北医科大学