Diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs

作者：Hodges Joanne; Christopher Mary M^*

来源：Journal of the American Veterinary Medical Association, 2011, 238(11): 1452-1458.

Objective-To determine diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs.
Design-Retrospective and prospective cross-sectional study.
Animals-4,521 anemic dogs.
Procedures-CBC results obtained between July 2002 and July 2008 by use of an automated laser-based flow cytometric hematology analyzer from dogs with Hct values <= 35% were retrieved. Sensitivity, specificity, accuracy, and predictive values of using erythrocyte indices and polychromasia to identify regeneration were determined, with a reticulocyte count > 65,000 reticulocytes/mu L considered the gold standard. Similarly, 134 blood samples from anemic dogs were analyzed prospectively with an in-house electrical impedance analyzer.
Results-Of 4,387 dogs with samples analyzed retrospectively, 1,426 (32.5%) had regenerative anemia. Of these, 168 (11.8%) had macrocytic hypochromic anemia. High mean cell volume and low mean cell hemoglobin concentration had low sensitivity (11%), high specificity (98%), and moderate accuracy (70%) when used to identify regenerative anemia. Use of polychromasia alone had an accuracy of 77%, and use of polychromasia combined with a high RBC distribution width (RDW) had an accuracy of 79%. Results obtained with the in-house analyzer were similar.
Conclusions and Clinical Relevance-Results suggested that most regenerative anemias in dogs were not macrocytic hypochromic. Polychromasia, with or without high RDW, was a more accurate indicator than other erythrocyte indices of regenerative anemia. To avoid a false diagnosis of nonregenerative anemia, a blood smear should be evaluated in anemic dogs when a reticulocyte count is not available. (J Am Vet Med Assoc 2011;238:1452-1458)

• 出版日期2011-6-1