Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

作者：Zhuang Zheng-Fei; Liu Han-Ping; Guo Zhou-Yi; Zhuo Shuang-Mu; Yu Bi-Ying; Deng Xiao-Yuan^*

来源：Chinese Physics B, 2010, 19(4): 049501.

DOI：10.1088/1674-1056/19/4/049501

• nonlinear microscopy
• multiharmonic generation
• diagnostic applications of nonlinear optics

This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Within a scanning region of 2.7 mu mx2.7 mu m in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL/6) have been investigated. Statistical analyses (t test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference (p < 0.05), suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.

• 出版日期2010-4
• 单位福建师范大学; 华南师范大学