Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

作者：Fairfoul, Graham; McGuire, Lynne I.; Pal, Suvankar; Ironside, James W.; Neumann, Juliane; Christie, Sharon; Joachim, Catherine; Esiri, Margaret; Evetts, Samuel G.; Rolinski, Michal; Baig, Fahd; Ruffmann, Claudio; Wade-Martins, Richard; Hu, Michele T. M.; Parkkinen, Laura; Green, Alison J. E.^*

来源：Annals of Clinical and Translational Neurology, 2016, 3(10): 812-818.

DOI：10.1002/acn3.338

• SLEEP BEHAVIOR DISORDER
• PARKINSONS-DISEASE
• CEREBROSPINAL-FLUID
• BIOMARKERS
• COHORT

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

• 出版日期2016-10