A functional genomic analysis of Arabidopsis thaliana PP2C clade D

作者：Tovar Mendez Alejandro; Miernyk Jan A^*; Hoyos Elizabeth; Randall Douglas D

来源：Protoplasma, 2014, 251(1): 265-271.

DOI：10.1007/s00709-013-0526-7

• Confocal microscopy
• Data mining
• GFP fusions
• Localization
• Transcript profiling

In the reference dicot plant Arabidopsis thaliana, the PP2C family of P-protein phosphatases includes the products of 80 genes that have been separated into ten multi-protein clades plus six singletons. Clade D includes the products of nine genes distributed among three chromosomes (APD1, At3g12620; APD2, At3g17090; APD3, At3g51370; APD4, At3g55050; APD5, At4g33920; APD6, At4g38520; APD7, At5g02760; APD8, At5g06750; and APD9, At5g66080). As part of a functional genomics analysis of protein phosphorylation, we retrieved expression data from public databases and determined the subcellular protein localization of the members of clade D. While the nine proteins have been grouped together based upon primary sequence alignments, we observed no obvious common patterns in expression or localization. We found chimera with the GFP associated with the nucleus, plasma membrane, the endomembrane system, and mitochondria in transgenic plants.

• 出版日期2014-1