Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields

作者：Gaetani Roberto; Ledda Mario; Barile Lucio; Chimenti Isotta; De Carlo Flavia; Forte Elvira; Ionta Vittoria; Giuliani Livio; D'Emilia Enrico; Frati Giacomo; Miraldi Fabio; Pozzi Deleana; Messina Elisa; Grimaldi Settimio; Giacomello Alessandro^*; Lisi Antonella

来源：Cardiovascular Research, 2009, 82(3): 411-420.

DOI：10.1093/cvr/cvp067

• Clinical translation protocols
• ELF-EMF
• Ca(2 ) ion cyclotron resonance
• Cardiac regenerative medicine

Modulation of cardiac stem cell (CSC) differentiation with minimal manipulation is one of the main goals of clinical applicability of cell therapy for heart failure. CSCs, obtained from human myocardial bioptic specimens and grown as cardiospheres (CSps) and cardiosphere-derived cells (CDCs), can engraft and partially regenerate the infarcted myocardium, as previously described. In this paper we assessed the hypothesis that exposure of CSps and CDCs to extremely low-frequency electromagnetic fields (ELF-EMFs), tuned at Ca(2 ) ion cyclotron energy resonance (Ca(2 )-ICR), may drive their differentiation towards a cardiac-specific phenotype.
A significant increase in the expression of cardiac markers was observed after 5 days of exposure to Ca(2 )-ICR in both human CSps and CDCs, as evidenced at transcriptional, translational, and phenotypical levels. Ca(2 ) mobilization among intracellular storages was observed and confirmed by compartmentalized analysis of Ca(2 ) fluorescent probes.
These results suggest that ELF-EMFs tuned at Ca(2 )-ICR could be used to drive cardiac-specific differentiation in adult cardiac progenitor cells without any pharmacological or genetic manipulation of the cells that will be used for therapeutic purposes.

• 出版日期2009-6-1