A bifunctional Fe (III)-coordinated nanoprobe for electrochemical detection of sphingosine kinase 1 activity

作者：Gu, Shiyu; Gao, Tao; Yang, Yucai; Zhi, Jun; Li, Jinlong; Xiang, Yang^*; Wang, Keming^*; Yang, Jie^*

来源：Electrochemistry Communications, 2016, 72: 104-108.

DOI：10.1016/j.elecom.2016.09.015

• Bifunctional nanoprobe
• Coordinated Fe (III) ion
• Kinase
• Phosphorylation
• Lipid-simulating surface

Kinase analysis is of fundamental significance in biochemical research. Here, we report on an electrochemical method to improve the simplicity of kinase analysis taking advantage of a novel bifunctional Fe (III)-coordinated nanoprobe. The Fe (III)-immobilized nanoprobe is designed to be able not only to recognize phosphorylated sites with high specificity, but also to generate electrochemical signal through NTA-Fe (III) catalyzed H2O2 redox. In this way, the phosphorylation recognition and signal output can be realized by a one-off solution, which significantly improves the simplicity of the kinase assay. Moreover, owing to the enriched NTA-Fe (III) on each nanoprobe and the continuous catalytic ability of each NTA-Fe (III), this method ensures very high sensitivity by dual signal amplification. Taking sphingosine kinase 1 (SphK1) as a model kinase, a limit of detection as low as 7.404 pmol min(-1) mg(-1) is achieved while keeping the kinase in its lipid-simulating catalytic environment. As a result, this assay method can act as a sample method for lipid kinase detection, which may also hold great potential in clinical analysis of other vital kinases.

• 出版日期2016-11
• 单位南京大学; 南京医科大学