Assessment of cholinesterase and tyrosinase inhibitory and antioxidant properties of Viscum album L. samples collected from different host plants and its two principal substances

作者：Orhan Didem Deliorman; Senol Fatma Sezer; Ho**as Sanem; Orhan Ilkay Erdogan^*

来源：Industrial Crops and Products, 2014, 62: 341-349.

DOI：10.1016/j.indcrop.2014.08.044

• Viscum album
• Loranthaceae
• Cholinesterase
• Tyrosinase
• Antioxidant activity

In the current study, the aqueous, dichloromethane, and methanol extracts of 12 samples of Viscum album L. collected on different host plants as well as two isolated compounds [syringin (VA-1) and 5,7-dimethoxyflavanone-4 %26apos;-O-[5 %26apos;%26apos;%26apos;-O-trans-cinnamoyl-beta-D-apiofuranosyl]-[beta-D-glucopyranoside (VA-2)] were tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tryosinase (TYRO) enzymes at 2000 mu g/mL by ELISA microtiter plate assays. Antioxidant activity of the extracts and compounds was evaluated by six in vitro methods [2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), and nitric oxide radical scavenging assays, metal-chelation capacity, ferric-(FRAP), and phosphomolibdenum-reducing antioxidant power (PRAP)]. Total phenolic and flavonoid contents of the extracts were determined using Folin-Ciocaltaeu%26apos;s and AlCl3 reagents, respectively. None of the extracts had any marked inhibition against tyrosinase, while they exerted a low inhibition against AChE and BChE at 200 mu g/mL. The aqueous and methanol extracts displayed a strong antioxidant activity in DPPH and DMPD radical quenching tests as well as FRAP and metal-chelation capacity assays.

• 出版日期2014-12