Degenerate primers based RT-PCR for rapid detection and differentiation of airborne chicken Newcastle disease virus in chicken houses

作者：Li Xiaoxia; Qu Yuyu; Yu Ailian; Chai Tongjie^*; Zhang Xingxiao; Liu Jinhua; Wang Decai; Wang Hairong; Wang Zhiliang; Song Cuiping

来源：Journal of Virological Methods, 2009, 158(1-2): 1-5.

DOI：10.1016/j.jviromet.2009.01.011

• Airborne Newcastle disease virus
• All Glass Impinger-30 (AGI-30)
• Degenerate primers
• Reverse transcription polymerase chain reaction (RT-PCR)

Airborne Newcastle disease (ND) viruses in the air of five chicken houses were detected and differentiated by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers. Fifteen air samples were collected with All Glass Impinger-30 (AGI-30) air samplers in each house. Airborne ND viruses were also isolated and virulence identified by in vivo tests. Avirulent viruses were detected both in air samples and swab samples in four houses by degenerate primers based RT-PCR. Virulent viruses were detected only in the air samples by degenerate primers based RT-PCR in two houses. Seven strains viruses were isolated from the RT-PCR positive air samples. Of the seven strains, three strains were virulent viruses and four strains were avirulent viruses identified by in vivo tests. The results showed that it was feasible to detect and differentiate NDV in the air samples using degenerate primers based RT-PCR. This technique could decrease the time it required identify NDV infected flocks while distinguishing between virulent and avirulent viruses. It will help effectively to control Newcastle disease.

• 出版日期2009-6
• 单位山东农业大学; 山东第一医科大学; 中国动物卫生与流行病学中心