Activation of the Retroviral Budding Factor ALIX

作者：Zhai Qianting; Landesman Michael B; Chung Hyo Young; Dierkers Adam; Jeffries Cy M; Trewhella Jill; Hill Christopher P^*; Sundquist Wesley I

来源：Journal of Virology, 2011, 85(17): 9222-9226.

DOI：10.1128/JVI.02653-10

The cellular ALIX protein functions within the ESCRT pathway to facilitate intralumenal endosomal vesicle formation, the abscission stage of cytokinesis, and enveloped virus budding. Here, we report that the C-terminal proline-rich region (PRR) of ALIX folds back against the upstream domains and auto-inhibits V domain binding to viral late domains. Mutations designed to destabilize the closed conformation of the V domain opened the V domain, increased ALIX membrane association, and enhanced virus budding. These observations support a model in which ALIX activation requires dissociation of the autoinhibitory PRR and opening of the V domain arms.

• 出版日期2011-9