Analysis of the shedding of three beta-herpesyiruses DNA in Polish patients subjected to allogeneic hematopoietic stem cell transplantation: Six-year follow up

作者：Dzieciatkowski Tomasz; Tomaszewska Agnieszka^*; Przybylski Maciej; Rusicka Patrycja; Basakd Grzegorz W; Jedrzejczak Wieslaw W; Wroblewska Marta; Halaburda Kazimierz

来源：Journal of Clinical Virology, 2016, 76: 30-35.

DOI：10.1016/j.jcv.2016.01.005

• Hematopoietic stem cell transplantation
• Post-transplant infections
• beta-Herpesviruses
• Molecular diagnostics
• Real-time PCR

Background: Infections with human beta-herpesviruses are common worldwide and are still frequent in patients after hematopoietic stem cell transplantation. Some data suggest that HHV-6 and HHV-7 could take part in CMV reactivation from latency and/or progression of CMV disease in immunosupressed patients.
Objectives: The aims of this study were: (1) to summarise retrospectively the results of beta-herpesviruses DNA detection in a large group of adult allogeneic haematopoietic stem cell transplant recipients; and (2) to find a potential correlation between viruses belonging to this subfamily.
Study design: AlloHSCT recipients (N = 142) were examined in the early post-transplant period (median = 89 days). The presence of CMV, HHV-6 and HHV-7 was confirmed through detection and quantification of viral DNA, isolated from 1679 sera samples.
Results: CMV DNA alone was detected in 23.9% of patients, while single HHV-6 and HHV-7 were detected in 14.8% and 9.9% of individuals, respectively. The reactivation of more than one virus was identified in 31% of analysed patients. In cases of concurrent infection, HHV-7 was detected at the same time as HHV-6, and both of them were usually reactivated before CMV. The kinetics of virus reactivation and measured viral load may suggest a potential role of HHV-6 and HHV-7 as co-factors in CMV reactivation.
Conclusions: The observed kinetics of virus reactivation may strongly suggest a potential role of HHV-6 and/or HHV-7 as co-factors of CMV reactivation. The co-infection with these beta-herpesviruses could predispose patients after hematopoietic stem cell transplantation to a longer and more severe CMV infection.

• 出版日期2016-3