Ultrasound-Assisted Rapid Extraction of Bacterial Intracellular Medium-Chain-Length Poly(3-Hydroxyalkanoates) (mcl-PHAs) in Medium Mixture of Solvent/Marginal Non-solvent

作者：Ishak K A; Annuar M S M^*; Heidelberg T; Gumel A M

来源：Arabian Journal for Science and Engineering, 2016, 41(1): 33-44.

DOI：10.1007/s13369-015-1833-4

• Downstream processing
• Ultrasonication
• Organic solvent
• Medium-chain-length PHA
• Rapid extraction

Intracellular medium-chain-length poly(3-hydroxyalkanoates) (PHAs) produced by Pseudomonas putida Bet001 were extracted in an ultrasound-assisted process. A mixture of acetone (solvent) and heptane (marginal non-solvent) was used as the extraction medium. The effects of volumetric energy dissipation, extraction medium ratio and irradiation time on the extraction process were investigated. Sonication frequency of 37 kHz and heptane as marginal non-solvent facilitated the process. Following optimization, high PHA extraction rate of 74 x 10(-3)g PHA g(-1) dried biomass min(-1) was observed at ultrasonic energy output of 1151 +/- 3 J ml(-1) with 50: 50 solvent/marginal nonsolvent ratio for irradiation time of 5 min. PHA showed good stability under the rapid extraction process.

• 出版日期2016-1