科研之友
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摘要
Methods. aEuro integral A total of 28 ARKO mice (ARKO group) screened by Cre-lox and 28 male Wistar mice without ARKO (controlled group) were enrolled in our study. Route pathology was performed and p63 examination was detected by immunohistochemistry in testes. Linear correlations were used to explore potential associations between p63 protein expression and spermatogenic TMS score). Results. aEuro integral In ARKO group, inner diameter of seminiferous tubules was decreased (62 +/-+/- 1.3 mu mu m vs. 91 +/-+/- 1.2 mu mu m), thickness of the basal membrane of the tubules (4 +/-+/- 0.3 mu mu m vs. 2.7 +/-+/- 0.5 mu mu m), cellular population within tubules was reduced (2 +/-+/- 0.4 vs. 4 +/-+/- 0.1 layers), degree of spermatogenesis within the tubules turned to disturbance (3 +/-+/- 1.0 vs. 5 +/-+/- 0.1), Testicular Makler score was lower than controlled group (7 +/-+/- 0.2 vs.15 +/-+/- 0.3), they had significant differences (p < 0.01). P63 expressed significantly lower in ARKO group than that in Wistar group, and was limited at stages from spermatocyte to round spermatid. (Percentage of positive cells ? 68.1 +/-+/- 3.7 vs. 81.7 +/-+/- 5.1, p < 0.001). The HSCORE yielded similar results (HSCORE 3.7 +/-+/- 0.3 vs. 2.0 +/-+/- 0.2, p < 0.001). p63 protein expression was significantly positively correlated with spermatogenic r == 0.87, p < 0.01). Conclusions. aEuro integral p63 developed important effect on spermatogenesis and the regulatory effect of p63 on spermatogenesis mainly occurred in the early stage of spermiogenesis in testis.
- 出版日期2011-3
- 单位上海交通大学
