Aberrant expression of B-catenin, Pin1 and cylin D1 in salivary adenoid, cystic carcinoma: Relation to tumor proliferation and metastasis

作者：Zhou Chuan Xiang; Gao Yan^*

来源：Oncology Reports, 2006, 16(3): 505-511.

• Pin1
• beta-catenin
• cyclin D1
• immunohistochemistry
• Western blot
• RT-PCR
• salivary adenoid cystic carcinomas

The aims of this study were to investigate the expression levels of beta-catenin, Pin1 and cyclin D1 in salivary adenoid cystic carcinomas (SACC) and to evaluate its clinical importance, furthermore, to elucidate whether B-catenin expression was aberrant in SACC and whether Pin1 was involved in aberrant beta-catenin and cyclin D1 expression. The expression of Pin 1, B-catenin and cyclin D I were examined in the specimens of 65 patients with SACC by immunohistochemistry, protein and mRNA expressions were detected by Western blotting and RT-PCR in four SACC cell lines. Pin1 was overexpressed in 51 cases of SACC (78%), and high levels of Pin1 expression correlated with cyclin D1 positive expression (p=0.02). Fourteen (22%) cases showed positive immunoreactivity for B-catenin protein in the nuclear/cytoplasmic fraction in tumor tissues, which was defined as cytoplasm/nucleus staining, among which quite evident nuclear expression of beta-catenin was detected in six cases (9%), while cyclin D1 positive expression was detected in 41 cases of SACC (63%). Reduced membranous expression of beta-catenin was detected in the cases with metastasis (11/14). Theses results suggest that Pin1 and Wnt signalling pathway are activated in SACC and may play a pivotal role in SACC carcinogenesis and metastasis.

• 出版日期2006-9
• 单位北京大学