Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters

作者：Hostein Richard; Aouani Heykel; Rigneault Herve; Wenger Jerome^*

来源：Optics Letters, 2011, 36(17): 3317-3319.

DOI：10.1364/OL.36.003317

Conventional fluorescence detection is sensitive to an intricate product of the number of fluorescent emitters times the local excitation intensity. Here, we describe a method to locally quantify the excitation intensity and the number of emitters separately, enabling a clear distinction between the phenomena responsible for a given fluorescence signal. Our technique is based on harmonic excitation modulation and higher-order fluorescence demodulation. It is compatible with a wide range of observations, opening new characterization routes for fluorescence detection or imaging on complex systems, such as plasmonic substrates or scattering media.

• 出版日期2011-9-1