科研之友
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摘要
Two fission yeast temperature-sensitive mutants, Cut6 and Isn1, show a defect in nuclear division. The daughter nuclei differ dramatically in size (the phenotype designated Isd, large and small daughter), Fluorescence in situ hybridization (FISH) revealed that sister chromatids were separated in the Isd cells, but appeared highly compact in one of the two daughter nuclei. EM showed asymmetric nuclear elongation followed by unequal separation of nonchromosomal nuclear structures in these mutant nuclei. The small nuclei lacked electron-dense nuclear materials and contained highly compacted chromatin, The cut6(+) and Isd1(+) genes are essential for viability and encode, respectively, acetyl CoA carboxylase and fatty acid synthetase, the key enzymes for fatty acid synthesis. Gene disruption of Isd1(+) led to the Isd phenotype. Palmitate in medium fully suppressed the phenotypes of Isd1. Cerulenin, an inhibitor for fatty acid synthesis, produced the Isd phenotype in wild type, The drug caused cell inviability during mitosis but not during the G2-arrest induced by the cdc25 mutation, A reduced level of fatty acid thus led to impaired separation of nonchromosomal nuclear components. We propose that fatty acid is directly or indirectly required for separating the mother nucleus into two equal daughters.
- 出版日期1996-8
