Determination of Captopril in Biofluids by Luminol-Chloroauric Acid-Lysozyme Chemiluminescence

作者：Liu, Xiaoqing; Li, Yajuan; Song, Zhenghua^*

来源：Instrumentation Science & Technology, 2015, 43(2): 197-213.

DOI：10.1080/10739149.2014.979494

• pharmacokinetic
• chloroauric acid
• chemiluminescence
• flow injection
• captopril
• lysozyme

A rapid and sensitive method for determining captopril by flow injection chemiluminescence is reported. It was found that captopril quenched the chemiluminescence of the luminol-chloroauric acid-lysozyme system. The decreased chemiluminescence was linear with the logarithm of captopril concentration between 0.1 and 50.0ng center dot mL(-1) with a detection limit of 0.03ng center dot mL(-1) (3 sigma). At a flow rate of 2.0mL center dot min(-1), one analysis cycle including sampling and washing was accomplished within 36s. The chemiluminescence method was successfully applied to the continuous monitoring of captopril in human urine after oral intake over a period of 7.5hr. Captopril reached a maximum concentration in urine at 1.5hr and the total elimination ratio was 48.70 +/- 0.09%. The pharmacokinetic parameters of absorption rate constant (2.81 +/- 0.05h(-1)), elimination rate constant (0.88 +/- 0.02h(-1)), and elimination half-life time (0.79 +/- 0.01h) were obtained. The possible chemiluminescence mechanism of lysozyme/captopril reaction was obtained with molecular docking methods.

• 出版日期2015-3-4
• 单位西北大学