DETERMINATION OF AMOXICILLIN, AMPICILLIN AND PENICILLIN G USING A FLOW INJECTION ANALYSIS METHOD WITH CHEMILUMINESCENCE DETECTION

作者：Chivulescu Alexandru Ioan; Doni Mihaela Badea^*; Cheregi Mihaela Carmen; Danet Andrei Florin

来源：Romanian Journal of Chemistry, 2011, 56(3): 247-+.

• flow injection analysis
• chemiluminescence
• beta-lactam antibiotics analysis
• luminol
• UV irradiation

The chemiluminescence (CL) reactions coupled with flow injection techniques can provide good solutions for development of cheap and fast methods for antibiotic determination. In this work, three beta-lactam antibiotics, namely: amoxicillin, ampicillin and penicillin G, were determined by using a flow injection analysis (FIA) method based on CL reaction of beta-antibiotics with luminol in basic environment, in presence of potassium ferricyanide and ferrocyanide. The novelty of the method is represented by 10 to 350-fold enhancement of the analytical signal using an on-line UV irradiation step of the injected sample. The flow injection system parameters were studied and optimized (concentrations of luminol, Na(2)CO(3), K(3)[Fe(CN)(6)] and K(4)[Fe(CN)(6)], injected sample volume and total flow rate). Ampicillin and penicillin G were determined between 0.05 and 100 ppm and amoxicillin in the 0.25 - 100 ppm range. The developed method proved to be simple, cheap, reproducible and fast due to the UV irradiation of the analyzed samples that determine a lower detection limit in comparison with similar chemiluminometric methods reported in literature. Samples of pharmaceuticals were analyzed and applying the recovery test good results were obtained.

• 出版日期2011-3