摘要

In order to protect the feed chain from contamination by bovine spongiform encephalopathy (BSE), it is necessary to detect the content of cattle and sheep meat and bone meal (MBM) in poultry or pig MBM. The objective of this study was to investigate the feasibility to determine the content of cattle and sheep MBM in poultry or pig MBM by using near infrared reflectance spectroscopy (NIRS). The MBM specimens were collected from different areas of China. Two hundred samples were prepared by poultry or pig MBM deliberately adulterated with cattle or sheep MBM over the weight range 0 similar to 48%. Three regression methods were compared. (modified partial least squares, partial least squares and principal component regression). Three optimum-models were developed from calibration sets of poultry, pig and both, respectively. The coefficients of determination (R-2) and the relative prediction deviation (RPD) of independent validation sets were greater than 0.90, 3.0, respectively. It is concluded that NIBS can be used as a rapid method to detect the content of cattle and sheep MBM in poultry or pig MBM.