科研之友
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摘要
In soybean [Glycine max (L.) Merr.], mutations in genes involved in meiosis can lead to altered chromosome pairing and result in nonfunctional gametes. Mutability of the w(4) flower color locus in soybean is due to an unstable allele designated w(4)-m (mutable). Several germinal revertant studies using the w(4)-m system resulted in generation of mutants for necrotic roots, chlorophyll-deficiency, and sterility. In the present study, six male-sterile, female-sterile mutant lines were identified from an independent mutational event that involved T366H (female-partial sterile mutant), which was a germinal revertant of w(4)-m. In addition, two spontaneous mutations were identified that resulted in male-sterile, female-sterile mutants. The objectives of this study were to investigate if the newly identified six male-sterile, female-sterile germinal revertant mutants were allelic to previous germinal revertant steriles or are novel mutants and to molecularly map the locations of these six mutants along with two spontaneous male-sterile, female-sterile mutants. Three of the six mutants identified in the germinal revertant study mapped to the st8 region on chromosome Gm16 (molecular linkage group [MLG] J). The other three mutants mapped to a novel location on chromosome Gm14 (MLG B2). Of the two spontaneous mutants, one mapped to chromosome Gm02 (MLG D1b) and the second one mapped to Gm18 (MLG G).
- 出版日期2011-2
