Advantages of the doxorubicin-resistant, acute myelogenous leukemia cell line, AML-2/DX100, for the screening of multidrug resistance protein inhibitors and pro-oxidants

作者：Min YD; Yoo JC; Choi CH^*

来源：Biotechnology Letters, 2002, 24(16): 1327-1330.

DOI：10.1023/A:1019883931387

• catalase
• multidrug resistance protein
• pro-oxidant
• reactive oxygen species

The doxorubicin-resistant, acute myelogenous leukemia cell line, AML-2/DX100, characterized by the over-expression of multidrug resistance protein (MRP) and the down-regulation of catalase, has advantages for the screening of MRP inhibitors as well as for cytotoxic substances producing potential reactive oxygen species. The screening power of AML-2/DX100 cells for an MRP inhibitor, probenecid, was approximately 4-fold stronger than that of another resistant cell line, HL-60/Adr, over-expressing MRP. AML-2/DX100 was approximately 2- to 5-fold more sensitive to pro-oxidants such as Paraquat, H2O2 and t-butyl hydroperoxide, when compared with its parental cells.

• 出版日期2002-8