A multi-pumping flow system for chemiluminometric determination of ascorbic acid in powdered materials for preparation of fruit juices

作者：Pires CK; Lavorante AF; Marconi LMT; Meneses SRP; Zagatto EAG^*

来源：Microchemical Journal, 2006, 83(2): 70-74.

DOI：10.1016/j.microc.2006.02.001

• flow analysis
• multi-pumping
• ascorbic acid
• chemiluminescence
• fruit juices

A multi-pumping flow-based procedure with chemiluminescent detection is proposed for the determination of ascorbic acid, AA, in fruit juices (powdered form). The method relies on the inhibitory effect of AA on the oxidation of luminol by hydrogen peroxide in alkaline medium. The system comprises several discretely actuated solenoid pumps as the only active components. It handles 100 samples per hour, and requires 96 mu l of sample, 42 mu g of luminol and 105 mu g of potassium hexacyanoferrate(III) per determination. The analytical curve is linear up to about 11 mmol l(-1) AA, and detection limit is 0.17 mmol l(-1) AA. The system yields precise measurements (r.s.d.< 1%; n = 11), and recovery ranges from 94% to 106%. Results are in agreement with the reference method (AOAC) at the 95% confidence level.

• 出版日期2006-7