Use of a Baculovirus-Expressed Structural Protein for the Detection of Antibodies to Foot-and-Mouth Disease Virus Type A by a Blocking Enzyme-Linked Immunosorbent Assay

作者：Ko Young Joon^*; Lee Hyang Sim; Jeoung Hye Young; Heo Eun Jeong; Ko Hyo Rim; Chang Byung Sik; Joo Hoo Don; Gerelmaa U; Dashzeveg B; Tserendorj S; Sodnomdarjaa R; Park Jong Hyeon; Kweon Chang Hee; Cho In Soo; Paik Sang Gi

来源：Clinical and Vaccine Immunology, 2010, 17(1): 194-198.

DOI：10.1128/CVI.00374-09

A blocking enzyme-linked immunosorbent assay (ELISA) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with a cutoff of 53% inhibition. Its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking ELISA, indicating its potential as an alternative assay.

• 出版日期2010-1