Determination of Citrinin Using Molecularly Imprinted Solid Phase Extraction Purification, HPLC Separation, and Fluorescence Detection

作者：Appell Michael^*; Jackson Michael A; Wang Lijuan C; Bosma Wayne B

来源：Journal of Liquid Chromatography & Related Technologies, 2015, 38(20): 1815-1819.

DOI：10.1080/10826076.2015.1113546

• food safety
• HPLC
• MIP
• MISPE
• molecularly imprinted polymer
• mycotoxin

A liquid chromatography based method to detect citrinin in corn was developed using molecularly imprinted solid phase extraction (MISPE) sample clean-up. Molecularly imprinted polymers were synthesized using 1,4-dihydroxy-2-naphthoic acid as the template and an amine functional monomer. Density functional calculations suggest the mimic template interacts with the functional monomer in a similar manner as citrinin. Freundlich isotherm analysis indicated the template provided a significant imprinting effect for citrinin binding. A high performance liquid chromatography with fluorescence detection (HPLC-FD) method to detect citrinin in maize was developed utilizing the imprinted polymers for sample clean-up (excitation at 330nm; emission at 500nm). Recoveries of citrinin in spiked corn samples (0.03-3 mu gg(-1)) were between 82.3-91.5%. This study demonstrates that molecularly imprinted polymers are applicable in the solid phase extraction clean-up of corn samples for citrinin determination by HPLC-FD.

• 出版日期2015-12-14