Measurements of CD56+cells in peripheral blood and endometrium by flow cytometry and immunohistochemical staining in situ

作者：Laird S M^*; Mariee N; Wei L; Li T C

来源：Human Reproduction, 2011, 26(6): 1331-1337.

DOI：10.1093/humrep/der104

• uNK cells
• endometrium
• recurrent miscarriage
• CD56+

Background: CD56+ cells in peripheral blood or the endometrium may be increased in women with reproductive failure. However, the relationship between numbers of peripheral blood CD56+ and endometrial CD56+ cells is uncertain. The aim of this study was (i) to compare the numbers of CD56+ cells in peripheral blood and endometrium in samples taken simultaneously and (ii) to compare measurements by flow cytometry and immunohistochemistry of CD56+ cells in the same endometrial biopsy.
Method: Endometrial biopsies and blood were obtained from women with recurrent miscarriage (n = 25) on days LH+ 7-LH+ 9 of the cycle. The total number of CD56+, CD56+ CD16- and CD56+ CD16+ cells in blood was measured by flow cytometry; the number of CD56+ cells in the endometrium was determined by immunohistochemistry. Endometrial samples were also obtained from fertile women (n 20) and used to measure CD56+ and CD45+ cells, by both flow cytometry and immunostaining.
Results: There was no correlation between the numbers of total CD56+, CD56+ CD16- or CD56+ CD16+ in peripheral blood and the number of endometrial CD56+ cells in the same women. In endometrium from fertile women, a significant correlation was found between the numbers of CD56+ cells measured by flow cytometry and immunohistochemistry (correlation = 0.497, P = 0.026, when expressed as % total cells; correlation = 0.570, P = 0.009 when expressed as % CD45+ cells).
Conclusions: Measurements of CD56+ cells in peripheral blood do not correlate with endometrial CD56+ cell numbers and therefore should not be extrapolated to events in the endometrium. In contrast, measurements of endometrial CD56+ cells by flow cytometry and immunostaining correlate well.

• 出版日期2011-6