All-in-one dry-reagent time-resolved immunofluorometric assay for the rapid detection of HIV-1 and-2 infections

作者：Talha Sheikh M; Juntunen Etvi; Salminen Teppo; Sangha Amninder; Vuorinen Tytti; Khanna Navin; Pettersson Kim

来源：Journal of Virological Methods, 2015, 226: 52-59.

DOI：10.1016/j.jviromet.2015.10.004

• All-in-one
• Time-resolved fluorometry
• Immunoassay
• HIV
• Diagnostics
• Europium
• HIV antibody
• Testing

An all-in-one (AIO) dry-reagent time-resolved fluorometric immunoassay that requires minimal liquid handling was developed for the detection of anti-HIV-1 and -2 antibodies. To prepare the AIO wells, in vivo biotinylated capture antigens (r-Bio-HIV-1 env and r-Bio-HIV-2env) were immobilized on streptavidin-coated microtitration wells and Eu(III) chelate labelled non-biotinylated tracer antigens [r-HIV-1env-Eu(III) and r-HIV-2env-Eu(III)] were dried in stable form in the same wells. The HIV MO assay was evaluated with serum/plasma samples (n = 148) from in-house and commercial panels at two different incubation times of 15 min and 1 h. The overall sensitivity of the AIO assay was 98.6% and specificity was 100% for both the incubation times. The AIO assay can accept whole blood matrix. This assay is envisioned to fill the gap between the rapid point-of-care assays and traditional enzyme immunoassays (EIA) in terms of complexity and turnaround time, without compromising the performance.

• 出版日期2015-12-15