Arp2/3 Complex Is Required for Macrophage Integrin Functions but Is Dispensable for FcR Phagocytosis and In Vivo Motility

作者：Rotty Jeremy D; Brighton Hailey E; Craig Stephanie L; Asokan Sreeja B; Cheng Ning; Ting Jenny P; Bear James E^*

来源：Developmental Cell, 2017, 42(5): 498-+.

DOI：10.1016/j.devcel.2017.08.003

The Arp2/3 complex nucleates branched actin, forming networks involved in lamellipodial protrusion, phagocytosis, and cell adhesion. We derived primary bone marrow macrophages lacking Arp2/3 complex (Arpc2(-/-)) and directly tested its role in macrophage functions. Despite protrusion and actin assembly defects, Arpc2(-/-) macrophages competently phagocytose via FcR and chemotax toward CSF and CX3CL1. However, CR3 phagocytosis and fibronectin haptotaxis, both integrin-dependent processes, are disrupted. Integrin-responsive actin assembly and alpha M/beta 2 integrin localization are compromised in Arpc2(-/-) cells. Using an in vivo system to observe endogenous monocytes migrating toward full-thickness ear wounds we found that Arpc2(-/-) monocytes maintain cell speeds and directionality similar to control. Our work reveals that the Arp2/3 complex is not a general requirement for phagocytosis or chemotaxis but is a critical driver of integrin-dependent processes. We demonstrate further that cells lacking Arp2/3 complex function in vivo remain capable of executing important physiological responses that require rapid directional motility.

• 出版日期2017-9-11