Atmospheric Pressure Photo Ionization Hydrogen/Deuterium Exchange Mass Spectrometry-a Method to Differentiate Isomers by Mass Spectrometry

作者：Ahmed Arif; Kim Sunghwan^*

来源：Journal of the American Society for Mass Spectrometry, 2013, 24(12): 1900-1905.

DOI：10.1007/s13361-013-0726-6

• Mass spectrometry
• Hydrogen deuterium exchange
• Atmospheric pressure photo ionization
• Isomers

In this report, a method for in-source hydrogen/deuterium (H/D) exchange at atmospheric pressure is reported. The method was named atmospheric pressure photo ionization hydrogen/deuterium exchange mass spectrometry (APPI HDX MS). H/D exchange was performed by mixing samples dissolved in toluene with CH3OD solvent and analyzing the mixture using atmospheric pressure photo ionization mass spectrometry (APPI-MS). The APPI HDX spectra obtained with contact times between the analyte solution and methanol-OD (CH3OD) of %26lt; 0.5 s or 1 h showed the same pattern of H/D exchange. Therefore, it was concluded that APPI HDX occurred in the source but not in the solution. The proposed method does not require a specific type of mass spectrometer and can be performed at atmospheric pressure. H/D exchange can be performed in any laboratory with a mass spectrometer and a commercial APPI source. Using this method, multiple H/D exchanges of aromatic hydrogen and/or H/D exchange of active hydrogen were observed. These results demonstrated that H/D exchange can be used to distinguish between isomers containing primary, secondary, and tertiary amines, as well as pyridine and pyrrole functional groups.

• 出版日期2013-12