Determination of Methadone in Biological Samples Using Liquid Phase Microextraction with Back Extraction Combined with LC

作者：Ebrahimzadeh Homeira^*; Khezri Ehsan; Kamarei Fahimeh

来源：Chromatographia, 2010, 72(3-4): 231-238.

DOI：10.1365/s10337-010-1636-4

• Column liquid chromatography
• Liquid phase microextraction with back extraction
• Methadone in plasma and urine

Liquid phase microextraction with back extraction (LPME-BE) combined with liquid chromatography-ultra violet (LC-UV) was applied for the extraction and determination of methadone in biological fluids. At the optimized conditions, an enrichment factor of 386 and detection limit (LOD) of 0.2 mu g L(-1) were obtained. The calibration curve was linear (r (2) = 0.989) in the concentration range of 0.6-1,000 mu g L(-1). Within-day relative standard deviation RSD (S/N = 3) and between-day RSD were 2.7 and 5.9%, respectively. The feasibility of the proposed method was evaluated by extraction and determination of methadone in plasma and urine samples and satisfactory results were obtained.

• 出版日期2010-8