摘要
Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) and (in situ) surface-enhanced (resonant) Raman scattering (SE(R)RS) were used as "quality control" tools during the construction of a model biofuel cell anode. The model anode was a layered structure formed by 4-hydroxythiophenol (HTP) attached to a coenzyme analog, cibacron blue F3G-A (CB), which then supported the adsorption of the enzyme formaldehyde dehydrogenase (FalDH). Ex situ SERS and PM-IRRAS showed the HTP attached to a gold surface through its thiol group. The covalent binding of CB to the HTP layer was confirmed by a combination of ex situ and in situ SERS data. In situ SERS spectra after adsorption of FaIDH to the CB layer confirmed successful enzyme immobilization.
- 出版日期2011-1-13