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摘要
An electrochemical drug-DNA biosensor was developed for the detection of interaction between the anti-cancer drug, Temozolomide (TMZ), and DNA sequences by using Differential Pulse Voltammetry at the graphite electrode surfaces. TMZ is a pro-drug and an alkylating agent that crosses the blood-brain barrier, so it is mainly used for brain cancers treatment. In this study, we aim to develop a-proof-of-concept study to investigate the effect of TMZ on formerly methylated DNA sequences since TMZ shows its anti-cancer activity by methylating the DNA. Interaction between TMZ and DNA causes localized distortion of DNA away from an idealized B-form, resulting in a wider major groove and greater steric accessibility of functional groups in the base of the groove. According to the results, TMZ behaves as a 'hybridization indicator' because of its different electrochemical behavior to different strands of DNA. After interaction with TMZ, hybrid (double stranded DNA-dsDNA) signals decreased dramatically whereas probe (single stranded DNA-ssDNA) and control signals remain almost unchanged. The signal differences enabled us to distinguish ssDNA and dsDNA without using a label or tag. It is the first study to demonstrate the interaction between the TMZ and dsDNA created from probe and target. We use specific oligonucleotides sequences instead of using long dsDNA sequences.
- 出版日期2016-5
