An aptamer-based fluorescence bio-sensor for chiral recognition of arginine enantiomers

作者：Yuan, Haiyan; Huang, Yunmei; Yang, Jidong^*; Guo, Yuan; Zeng, Xiaoqing; Zhou, Shang; Cheng, Jiawei; Zhang, Yuhui

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 200: 330-338.

DOI：10.1016/j.saa.2018.04.038

• Aptamer
• Bio-sensor
• Chiral recognition
• Arg enantiomers
• Logic gate

In this study, a novel aptamer - based fluorescence bio-sensor (aptamer-AuNps) was developed for chiral recognition of arginine (Arg) enantiomers based on aptamer and gold nanoparticles (AuNps). Carboxyfluorescein (FAM) labeled aptamers (Apt) were absorbed on AuNps and their fluorescence intensity could be significantly quenched by AuNps based on fluorescence resonance energy transfer (FRET). Once D-Arg or L-Arg were added into the above solution, the aptamer specifically bind to Arg enantiomers and released from AuNps, so the fluorescence intensity of D-Arg system and L-Arg system were all enhanced. The affinity of Apt to L-Arg is tighter to DArg, so the enhanced fluorescence signals of L-Arg system was stronger than D-Arg system. What's more, the enhanced fluorescence were directly proportional to the concentration of D-Arg and L-Arg ranging from 0-300 nM and 0-400 nM with related coefficients of 0.9939 and 0.9952, respectively. Furthermore, the method was successfully applied to detection L-Arg in human urine samples with satisfactory results. Eventually, a simple "OR" logic gate with D-Arg & L-Arg as inputs and AuNps aggregation state as outputs was fabricated, which can help us understand the chiral recognition process deeply.

• 出版日期2018-7-5
• 单位长江师范学院; 重庆三峡学院