科研之友
微信
新浪微博
Facebook
分享链接
摘要
Male sterility induced by chemical hybridization agents (CHAs) is a highly potential approach for heterosis in crop breeding. To investigate the effects of male sterility induced by SQ-1 in foxtail millet (Setaria italica), experiments were conducted with various SQ-1 concentrations and at different plant developmental stages of the foxtail millet Henggu 13 in 2015 and 2016. Some studies have suggested that CHA induced male sterility may suffer from oxidative stress. To study the physiological responses, the production and scavenging effects of the reactive oxygen species (ROS) were investigated. The results showed that the maximum male sterility was obtained at the early protogyny stage when a SQ-1 concentration of 5 kg ha(-1) was applied. The spikelets of the SQ-1 treated plants presented higher contents of H2O2 and MDA than those of untreated plants at all stages of the treatment. Moreover, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in scavenging ROS in the spikelets were down regulated at different SQ-1 concentrations when compared to the ones determined for the control plants. However, the SQ-1 treated spikelets presented a higher peroxidase (POD) concentration than the control plants, but the quantity was not enough to eliminate the produced ROS. In addition, the proline and soluble protein contents were both lower than the one of the control plants. In conclusion, the sterility could be induced by SQ-1 due to the chronic oxidative stress caused by the abnormal increase of ROS.
- 出版日期2017
- 单位山西农业大学
