Development of a rapid genetic technique for the identification of clupeid larvae in the Western English Channel and investigation of mislabelling in processed fish products

作者：Brechon Amanda L^*; Coombs Stephen H; Sims David W; Griffiths Andrew M

来源：ICES Journal of Marine Science, 2013, 70(2): 399-407.

DOI：10.1093/icesjms/fss178

• climate
• Clupea harengus
• Engraulis encrasicolus
• high-resolution melt analysis
• Sardina pilchardus
• species identification
• Sprattus sprattus

A novel genetic technique, involving real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) analysis was developed, which successfully identified four species of clupeid larvae from the Western English Channel and revealed the presence of herring (Clupea harengus). This was unexpected in a context of global warming as herring are at the southern limit of their range off southwest England, where the larvae are generally very rare. The application of the method in cases of degraded DNA, such as in processed fishery products, was also assessed, revealing a low level of misidentification or equivocal identity (8.3%). Eight-year-old formalin-preserved samples were also analysed successfully. This highlights the potentially broad applications for HRM-based approaches to species identification in marine science.

• 出版日期2013-3