Analysis of mitochondrial genomes in Citrus interspecific somatic hybrids produced by protoplast fusion

作者：Guo Wen Wu^*; Wu Reng Chao; Fan Gai En; Cheng Yun Jiang

来源：Botanical Studies, 2008, 49(4): 295-300.

• Citrus
• Cleaved amplified polymorphic sequence (CAPS)
• Cytoplasmic genome
• DNA sequencing
• Simple sequence repeat (SSR)
• Somatic hybrids

Somatic hybrids produced via protoplast fusion are valuable germplasm for citrus improvement. Herein, four randomly selected six-year-old interspecific somatic hybrid plants between Bonanza sweet orange (Citrus sinensis Osbeck) and rough lemon (C. jambhiri), were thoroughly analyzed. Flow cytometry and nuclear SSR analysis confirmed them as true tetraploid somatic hybrids. Chloroplast SSR analysis showed the random inheritance nature of chloroplast DNA. PCR amplification of mitochondrial genome by universal primer pair showed that the somatic hybrids had the specific band from rough lemon (the leaf parent) while all samples shared a common band. CAPS analysis by further TasI restriction endonuclease cut of the PCR products, however, revealed the band specific to Bonanza orange, the embryogenic callus parent,was also present in all these analyzed hybrids. Further sequencing of the common band and searching for restriction endonuclease recognition sites well explained the banding pattern by CAPS analysis. It was concluded that mitochondrial recombination in citrus somatic hybrids occurred as revealed by CAPS analysis and DNA sequencing.

• 出版日期2008-10
• 单位华中农业大学